Journal: Neural Plasticity

Article Title: Destructive Effects of Pyroptosis on Homeostasis of Neuron Survival Associated with the Dysfunctional BBB-Glymphatic System and Amyloid-Beta Accumulation after Cerebral Ischemia/Reperfusion in Rats

doi: 10.1155/2021/4504363

Figure Lengend Snippet: Effects of LPS and Gly interventions on expression levels of pyroptosis-related proteins and observations of pyroptosis locations. (a, b) Expression levels of pro-/cleaved caspase-11 and GSDMD-FL/N by Western blotting analysis, n = 6. (c) Representative pictures of the double immunofluorescence staining (white arrows) of caspase-11 (red) colocalized with Iba-1 or GFAP (green), respectively, scale bars, 50 μ m. (d, e) Expression levels of NLRP3, cleaved caspase-1, IL-6, and cleaved IL-1 β , n = 6. Data are presented as mean ± SD. ## P < 0.01, I/R group versus sham group; ▲ P < 0.05, ▲▲ P < 0.01, I/R + LPS group versus I/R group; ∗∗ P < 0.01, I/R + Gly group versus I/R group.

Article Snippet: Subsequently, the sections were incubated with the first antibodies mixed for caspase-11/ionized calcium-binding adapter molecule-1 (Iba-1) (#ab178847, Abcam), caspase-11/glial fibrillary acidic protein (GFAP) (#23935-1-AP, Proteintech), A β (#sc-28365, Santa Cruz)/GFAP, and GSDMD (#20770-1-AP, Proteintech)/GFAP (#60190-1-Ig, Proteintech) overnight at 4°C followed by incubations with corresponding mixed Alexa Fluor 488/647 secondary antibodies (1 : 500, #A0423/A0473, #A0428/A0468, Beyotime) for 1 h at room temperature.

Techniques: Expressing, Western Blot, Double Immunofluorescence Staining

Journal: Journal of Neuroinflammation

Article Title: Deficiency of macrophage migration inhibitory factor attenuates tau hyperphosphorylation in mouse models of Alzheimer’s disease

doi: 10.1186/s12974-015-0396-3

Figure Lengend Snippet: Upregulation of MIF in ICV-STZ mice and colocalization with GFAP in the hippocampus. a Serial sections of WT mouse brain were stained for MIF protein using a rabbit anti-MIF polyclonal antibody and Alexa Fluor 488-conjugated anti-rabbit IgG ( green fluorescence ). The sections were subsequently stained for GFAP using a monoclonal anti-GFAP Cy3 TM antibody ( red fluorescence ). Nuclei were stained with DAPI ( blue ). Images with combined fluorescent channels are shown at ×200 (scale bar in the upper left panel 75 μm). Selected areas are enlarged by 10 times and shown as combined as well as individual fluorescence stains. Quantification of the MIF ( b ) and GFAP ( c ) fluorescence was shown after normalization of the green (MIF) and red (GFAP) fluorescence against the blue fluorescence (nuclei). Data shown are means ± SEM based on multiple experiments ( n = 3). * p < 0.05, ** p < 0.01 compared with WT mice receiving saline

Article Snippet: GFAP , Poly- , GFAP , , Proteintech, Chicago, IL, USA.

Techniques: Staining, Fluorescence